As the 16S profiles within the gut appear pretty unique for intracellular and extracellular DNA [33], qPCR is predicted to mirror the NGS-targeted community structure the two quantitatively and qualitatively far more intently than stream cytometry.
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Even so, these variables Enjoy the same role during the NGS itself [34]. The fact that the qPCR-centered solution isn't going to introduce further biases to Those people already existing in NGS workflows could be Therefore regarded as a bonus.
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